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Gram-negative bacteria do not retain the crystal violet stain during Gram's staining method and instead absorb the red counterstain. This occurs because these bacteria have a cell wall made up of a thin layer of peptidoglycan. Many bacteria that typically inhabit the human body and can cause diseases, including gonococci and meningococci, are Gram-negative. Additionally, the pathogens responsible for diseases like bubonic plague also fall into this category. The Gram staining technique was invented by the Danish bacteriologist J.M.C. Gram (1853-1938) and utilizes a dye known as crystal violet. This method allows for the differentiation of bacteria based on their ability to retain the crystal violet stain, classifying them as either Gram-positive or Gram-negative.
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Understand how to safely and effectively operate an Automated External Defibrillator (AED) for adults, children, and infants.
Learn how to perform the Heimlich maneuver and clear airway obstructions caused by choking in both adults and infants.
Gain experience working as part of a team when more than one rescuer is available during an emergency.
Receive your AHA CPR Certification E-Card on the same day you complete the class and pass the exam.
CPR Certification Labs provides professional CPR and emergency training courses based on recognized industry standards.
